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目的:研究红花多糖的提取及含量测定,为开展红花多糖的抗肿瘤作用机制提供理想药物。方法:采用水提醇沉法从红花药材中提取并分离出红花粗多糖,用Sevage法除去蛋白质,过氧化氢脱色;采用苯酚-硫酸显色,用分光光度法测定多糖含量。结果:红花多糖的浓度与吸光度在5～50μg·mL-1呈良好的线性关系,回归方程:Y=0.01749X-0.03165,r=0.9994。多糖质量分数为96.048%。结论:含量测定方法操作简便,灵敏度高,稳定,重现性好。该方法可为开展红花多糖的抗肿瘤作用研究提供理想的实验药物。
Objective: To study the extraction and determination of safflower polysaccharides, and to provide an ideal medicine for the antitumor mechanism of safflower polysaccharides. Methods: The crude polysaccharides were extracted and separated from safflower medicinal materials by water extraction and alcohol precipitation method. The proteins were removed by Sevage method and decolorized by hydrogen peroxide. The content of polysaccharides was determined by spectrophotometry with phenol-sulfuric acid. Results: The concentration of safflower polysaccharide showed a good linear relationship with the absorbance at 5 ~ 50μg · mL-1. The regression equation was Y = 0.01749X-0.03165, r = 0.9994. Polysaccharide content of 96.048%. Conclusion: The content determination method is simple, sensitive, stable and reproducible. The method can provide an ideal experimental drug for the research on antitumor effect of safflower polysaccharide.