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本研究旨在探讨藤黄酸对高危组骨髓增生异常综合征(MDS)细胞的生长抑制作用及其机制。以人MDS-RAEB细胞株MUTZ-1细胞为研究对象,采用MTT法测定增殖抑制率,流式细胞术检测细胞凋亡及细胞周期变化,用RT-PCR检测bax/bcl-2基因表达。结果表明,藤黄酸对MUTZ-1细胞有生长抑制作用,在0.2-0.8μg/ml浓度范围内随着药物浓度的增加MUTZ-1细胞的增殖抑制率增高;流式细胞学检测发现,藤黄酸浓度0、0.4、0.6、0.8μg/ml引起的MUTZ-1凋亡率分别为(5±0.5)%、(13±0.5)%、(37±0.7)%和(56±0.6)%,而且凋亡率随着药物浓度增加而增加(p<0.05)。bcl-2基因表达程度随藤黄酸剂量增加而减弱,而baxmRNA表达无明显变化。结论:藤黄酸通过诱导MUTZ-1细胞凋亡、下调bcl-2基因表达而抑制该细胞生长,这可能是藤黄酸抗肿瘤的主要机制之一。
This study was designed to investigate the inhibitory effect of gambogic acid on high-risk myelodysplastic syndrome (MDS) cells and its mechanism. The MDS-RAEB cell line MUTZ-1 cells were used as the research object. MTT assay was used to determine the proliferation inhibition rate. Flow cytometry was used to detect cell apoptosis and cell cycle changes. The expression of bax/bcl-2 gene was detected by RT-PCR. The results showed that gambogic acid had a growth inhibitory effect on MUTZ-1 cells, and the proliferation inhibition rate of MUTZ-1 cells increased with increasing drug concentration in the concentration range of 0.2-0.8 μg/ml; flow cytometry revealed that The apoptotic rates of MUTZ-1 caused by the concentrations of folate at 0, 0.4, 0.6, and 0.8 μg/ml were (5±0.5)%, (13±0.5)%, (37±0.7)%, and (56±0.6)% respectively. , And the rate of apoptosis increased with increasing drug concentration (p<0.05). The expression level of bcl-2 gene was decreased with the increase of gambogic acid dose, but there was no significant change in bax mRNA expression. Conclusion: Gambogic acid can inhibit the growth of MUTZ-1 cells by inducing apoptosis and down-regulating the expression of bcl-2 gene, which may be one of the main mechanisms of anti-tumor activity of gambogic acid.