This paper focuses on the indicators of soil and litter health, disturbance, and landscape heterogeneity as a tool for prediction of ecosystem sustainability in the northern forests of Iran. The study area was divided into spatial homogenous sites using slope, aspect, and soil humidity classes. Then a range of sites along the disturbance gradient was selected for sampling. Chemical and physical indicators of soil and litter health were measured at random points within these sites. Structural equation modeling(SEM) was applied to link six constructs of landscape heterogeneity, three constructs of disturbance(harvest, livestock, and human accessibility), and soil and litter health. The results showed that with decreasing accessibility, the total N and organic matter content of soil increased and effective bulk density decreased. Harvesting activities increased soil organic matter. Therefore, it is concluded that disturbances through harvesting and accessibility inversely affect the soil health. Unexpectedly, it was found that the litter total C and C:N ratio improved with an increase in the harvest and accessibility disturbances, whereas litter bulk density decreased. Investigation of tree composition revealed that in the climax communities, which are normally affected more by harvesting activities, some species like Fagus orientalis Lipsky with low decomposition rate are dominant. The research results showed that changes in disturbance intensity are reflected in litter and soil indicators, whereas the SEM indicated that landscape heterogeneity has a moderator effect on the disturbance to both litter and soil paths. This paper focuses on the indicators of soil and litter health, disturbance, and landscape heterogeneity as a tool for prediction of ecosystem sustainability in the northern forests of Iran. The study area was divided into spatial homogenous sites using slope, aspect, and soil humidity classes . Then a range of sites along the disturbance gradient was selected for sampling. Chemical and physical indicators of soil and litter health were measured at random points within these sites. Structural equation modeling (SEM) was applied to link six constructs of landscape heterogeneity, three constructs of disturbance (harvest, livestock, and human accessibility), and soil and litter health. The results showed that with decreasing accessibility, the total N and organic matter content of soil increased and effective bulk density decreased. Harvesting activities increased soil organic matter. Therefore, it is said that disturbances through harvesting and accessibility inversely affect the soil health of Unexpectedly, it was found that the litter total C and C: N ratio improved with an increase in the harvest and accessibility disturbances, and litter bulk density decreased. Investigation of tree composition revealed that in the climax communities, which are normally affected more by harvesting activities, some species like Fagus orientalis Lipsky with low decomposition rate are dominant. The research results showed that changes in disturbance intensity are reflected in litter and soil indicators, but the SEM indicates that landscape heterogeneity has a moderator effect on the disturbance to both litter and soil paths.