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目的 :分析急性白血病的抗原表达及其临床意义。方法 :采用一组系列相关单抗直接免疫荧光标记 CD45设门的多参数流式细胞术 ,检测 35例急性白血病患者的免疫表型。结果 :11例 ALL中 B-AL L 8例 ,T-AL L3例 ,其中出现髓系抗原表达 4例 ,占 36 .4% ,CD3 4 表达 10例 ,占 90 .1% ;2 4例 AML 中伴淋系抗原表达 7例 ,占 2 9.17% ,CD3 4 表达 16例 ,占 70 .8% ,DR的表达与 CD3 4 一致 ,5例 M3 患者均无 CD3 4 和 HLA DR表达。伴髓系抗原表达的 AL L CR率低于髓系抗原阴性表达者 ( 1/3及 5 /6 ) ,但统计学上差异无显著性 ( P>0 .0 5 ) ;伴淋系抗原表达的 AML患者 CR率明显低于淋系抗原阴性表达者 ( 0 /5及 10 /10 ) ,两组间差异具有显著性 ( P<0 .0 1)。结论 :CD45设门的多参数流式细胞术是分析白血病免疫表型的最好方法 ,白血病抗原的错义表达是预后不良的因素之一
Objective: To analyze the expression of antigen in acute leukemia and its clinical significance. METHODS: A series of monoclonal antibodies were used to directly immunofluorescently label CD45-based multiparameter flow cytometry to detect the immunophenotype of 35 patients with acute leukemia. Results: Of the 11 cases of ALL, 8 were B-AL L and 3 were T-AL L. There were 4 cases of myeloid antigen expression, accounting for 36.4%, 10 cases of CD3 4 expression, accounting for 90.1%, and 24 cases of AML. There were 7 cases of lymphoid antigen expression in the colitis, accounting for 29.17%, and CD3 4 expression in 16 cases, accounting for 70.8%. The expression of DR was consistent with that of CD34. There were no CD3 4 and HLA DR expression in 5 cases of M3. The expression of AL LCR with myeloid antigen was lower than that with myeloid antigen negative expression (1/3 and 5/6), but there was no statistically significant difference (P>0.05); The CR rate of patients with AML was significantly lower than that of patients with negative lymphocytic antigen expression (0/5 and 10/10), and the difference between the two groups was significant (P < 0.01). Conclusion: CD45-based multiparameter flow cytometry is the best method to analyze the immunophenotype of leukemia. The missense expression of leukemia antigen is one of the factors that have poor prognosis.