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目的探讨粒细胞集落刺激因子(G-CSF)对百草枯干预小鼠脑内黑质纹状体通路的影响。方法 C57BL/6小鼠随机分为百草枯组、G-CSF干预组和对照组(均n=8)。高效液相色谱分析测定纹状体多巴胺含量,同时采用免疫组化ABC法观察黑质部酪氨酸羟化酶(TH)阳性表达神经元。结果腹腔注射百草枯20 mg·kg~(-1)·d~(-1),连续5 d后,小鼠自发性活动明显减少,纹状体多巴胺含量明显减少,黑质部TH阳性表达明显减少,与对照组比较差异有统计学意义(P<0.05);给予G-CSF干预后纹状体多巴胺含量和黑质部TH阳性表达明显增加,与百草枯组比较差异有统计学意义(P<0.05)。结论 G-CSF干预后小鼠纹状体的多巴胺含量升高,黑质部TH阳性表达增加,提示G-CSF对小鼠黑质纹状体通路多巴胺能神经元可能具有一定的保护作用。
Objective To investigate the effect of granulocyte-colony stimulating factor (G-CSF) on the nigrostriatal pathway in mice brain after paraquat treatment. Methods C57BL / 6 mice were randomly divided into paraquat group, G-CSF intervention group and control group (n = 8). The content of dopamine in the striatum was determined by HPLC, and tyrosine hydroxylase (TH) -positive neurons in substantia nigra were observed by immunohistochemical ABC method. Results Paraquat 20 mg · kg -1 · d -1 was injected intraperitoneally. After 5 days of continuous operation, the spontaneous activity of the mice decreased significantly, the content of dopamine in the striatum decreased significantly, and the positive expression of TH in substantia nigra was observed (P <0.05). After administration of G-CSF, dopamine levels in striatum and TH-positive cells in substantia nigra were significantly increased compared with those in control group (P <0.05). CONCLUSION: G-CSF increases the dopamine level in the striatum and the TH-positive expression in the substantia nigra at the G-CSF level, suggesting that G-CSF may have a protective effect on dopaminergic neurons in the nigrostriatal pathway in mice.