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目的探讨溃疡性结肠炎(UC)病人肠黏膜蛋白酶激活受体2(PAR-2)、核转录因子-κB(NF-κB)表达和肥大细胞的变化,以及三者在UC发病机制中的作用。方法32个黏膜标本取自行结肠镜检查的UC病人,并对UC的病理组织学炎症进行分级,Ⅰ、Ⅱ级12例,Ⅲ、Ⅳ级20例;对照组肠黏膜取自15名健康成人。半定量RT-PCR检测两组肠黏膜PAR-2的mRNA表达,免疫组化方法检测PAR-2、NF-κB p65蛋白表达和肥大细胞数量。结果与对照组相比,UC组肠黏膜中PAR-2 mRNA和蛋白过度表达,PAR-2 mRNA表达与疾病严重程度正相关(P<0.01)。免疫组化结果显示,UC组肠黏膜肥大细胞数显著高于对照组(P<0.01)。正常对照组肠黏膜无或微弱阳性PAR-2、NF-κB p65表达。病理分级Ⅲ、Ⅳ级肠黏膜PAR-2、NF-κB p65蛋白表达及肥大细胞数均较病理分级Ⅰ、Ⅱ级组明显增加(P<0.05)。PAR-2与肥大细胞数量之间呈正相关(r=0.78,P<0.01),PAR-2与NF-κB p65之间也呈正相关(r=0.56,P<0.01)。结论UC的发生发展与PAR-2、NF-κB p65的过度表达及肥大细胞数量变化密切相关。
Objective To investigate the changes of intestinal mucosal protease-activated receptor 2 (PAR-2), nuclear factor kappa B (NF-κB) expression and mast cells in patients with ulcerative colitis (UC) and their roles in the pathogenesis of UC . Methods Twenty-two mucosal specimens from UC patients undergoing colonoscopy were collected. Pathological inflammation of UC was graded. There were 12 cases of grade Ⅰ and Ⅱ, 20 cases of grade Ⅲ and Ⅳ. The control group received intestinal mucosa from 15 healthy adults . Semi-quantitative RT-PCR was used to detect the mRNA expression of PAR-2 in both groups. The expression of PAR-2, NF-κB p65 protein and the number of mast cells were detected by immunohistochemistry. Results Compared with the control group, the expression of PAR-2 mRNA and protein in UC group was significantly increased, and the expression of PAR-2 mRNA was positively correlated with the severity of disease (P <0.01). Immunohistochemical results showed that the number of intestinal mucosa mast cells in UC group was significantly higher than that in control group (P <0.01). The expression of PAR-2 and NF-κB p65 in intestinal mucosa of normal control group was no or weakly positive. Pathological grade Ⅲ, Ⅳ intestinal mucosa PAR-2, NF-κB p65 protein expression and mast cell number than the pathological grade Ⅰ, Ⅱ group significantly increased (P <0.05). There was a positive correlation between PAR-2 and the number of mast cells (r = 0.78, P <0.01). There was also a positive correlation between PAR-2 and NF-κB p65 (r = 0.56, P <0.01). Conclusion The occurrence and development of UC are closely related to the overexpression of PAR-2 and NF-κB p65 and the changes of the number of mast cells.