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目的:建立HPLC-ELSD法测定益心舒片中黄芪甲苷的含量。方法:采用HPLC-ELSD法,Shim-pack ODS色谱柱,乙腈-水(32∶68)为流动相,流速为1.0mL·min~(-1),柱温为25℃,漂移管温度为80℃,气体流速为1.5L·min~(-1),增益为2。结果:黄芪甲苷对照品在0.524~5.242μg范围内线性关系良好(r=0.999 8);平均回收率为101.8%,RSD为1.76%(n=6)。结论:建立的方法操作简便、准确、重复性好,可作为该制剂的含量测定方法。
Objective: To establish a HPLC-ELSD method for the determination of Astragaloside IV in YixinShu tablets. Methods: The mobile phase was acetonitrile-water (32:68) at a flow rate of 1.0 mL · min -1 using HPLC-ELSD with a Shim-pack ODS column. The column temperature was 25 ℃ and the drift tube temperature was 80 ℃, gas flow rate of 1.5L · min -1, gain of 2. Results: The calibration curve of Astragaloside IV was linear in the range of 0.524-5.242 μg (r = 0.999 8). The average recovery was 101.8% and the RSD was 1.76% (n = 6). Conclusion: The established method is simple, accurate and reproducible. It can be used as a method for determination of the content of the preparation.