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建立了LC-MS/MS法测定人血浆中的扎托布洛芬。以瑞舒伐他汀钙为内标,采用C_(18)色谱柱,以甲醇-5mmol/L乙酸铵溶液(70:30)为流动相,采用电喷雾电离源、选择反应监测(SRM)负离子扫描方式检测。扎托布洛芬在0 02～8μg/ml浓度范围内线性关系良好,日内和日间RSD均小于15%,回收率为77.5%～87.6%。24名健康志愿者单剂量口服扎托布洛芬80mg后的主药动学参数为:c_(max)(4.16±1.46)μg/ml,t_(max)(1.54±1.00)h,t_(1/2)(3.89±1.69)h,AUC_(0-∞)(15.20±3.01)μg·h·ml~(-1)。
The LC-MS / MS method was developed for the determination of zatoprofen in human plasma. Using rosuvastatin calcium as internal standard, C 18 column, methanol-5mmol / L ammonium acetate solution (70:30) as mobile phase, electrospray ionization source and selective reaction monitoring (SRM) Way to detect. Zatoprofen showed a good linearity in the range of 0 02 ~ 8μg / ml with daily RSD of less than 15% and daily RSD of 77.5% -87.6%. Twenty-four healthy volunteers received a single dose of oral zatoprofen 80 mg after the main pharmacokinetic parameters: c_ (max) (4.16 ± 1.46) μg / ml, t_ (max) (1.54 ± 1.00) h, t_ /2)(3.89 ± 1.69)h,AUC_(0-∞)(15.20 ±3.01)μg·h·ml~(-1).