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目的观察小鼠心肌梗死后骨髓造血干细胞在心脏内的分化及细胞因子的影响。方法C57/BL6小鼠60只分为骨髓动员组和对照组,先后行脾切除、骨髓移植(骨髓供体为增强绿色荧光蛋白转基因小鼠)、骨髓动员及建立心肌梗死模型。心肌梗死后3周将小鼠心脏取出并切片行组织学及激光共聚焦显微镜免疫荧光检查。结果骨髓动员可以增加EGFP阳性细胞在心脏中梗死区和边缘区的定植,但绝大多数EGFP阳性细胞都同时表达CD45。仅发现有极少数骨髓来源的心肌细胞、成纤维细胞及血管内皮细胞,且与骨髓动员无相关性。结论骨髓动员能够明显促进骨髓来源细胞定植入小鼠心脏的梗死区;极少数骨髓造血干细胞可以分化为心肌细胞,其数量远不足以修复梗死心肌及改善心功能;骨髓造血干细胞不参与梗死区疤痕形成的病理过程。
Objective To observe the intracardiac differentiation and cytokines of bone marrow hematopoietic stem cells after myocardial infarction in mice. Methods Sixty C57 / BL6 mice were divided into mobilization group and control group. Splenectomy, bone marrow transplantation (bone marrow donor enhanced green fluorescent protein transgenic mice), mobilization of bone marrow and establishment of myocardial infarction model were performed. Three weeks after myocardial infarction, the hearts of mice were removed and sectioned for histology and confocal laser scanning microscopy. Results Mobilization of bone marrow could increase the engraftment of EGFP positive cells in the infarct and marginal zone in the heart. However, the majority of EGFP positive cells expressed CD45 simultaneously. Only a few bone marrow-derived cardiomyocytes, fibroblasts and vascular endothelial cells were found, and there was no correlation with bone marrow mobilization. Conclusion Bone marrow mobilization can significantly promote bone marrow-derived cells to be implanted into the infarct region of mouse heart. A few bone marrow hematopoietic stem cells can differentiate into cardiomyocytes, the number is far from enough to repair myocardial infarction and improve cardiac function. Bone marrow hematopoietic stem cells do not participate in the infarction area Pathological process of scar formation.