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目的:对川崎病(KD)患儿血浆IL-10与T细胞亚群的相互关系进行研究。方法:选择30例KD患儿作为病例组,随机选择健康儿童23例作为对照组。应用S-P一步染色法及ELISA双抗体夹心法分别进行T细胞亚群和血浆IL-10浓度测定。结果:KD组急性期CD3~+、CD4~+细胞百分率均上升。但与恢复期和对照组比较差异无显著性意义(P>0.05);CD8~+细胞百分率明显降低,CD4~+/CD8~+值上升,与恢复期和对照组比较均差异有非常显著性(P<0.01)。血浆IL-10浓度在急性期显著上升,与恢复期、对照组比较差异有非常显著性意义(P<0.01)。冠状动脉病变(CAL)组较冠状动脉(CA)正常组急性期各项免疫指标改变更显著。血浆IL-10测值与CD8~+细胞百分率闭呈负相关性(r=-0.54,P<0.05)。结论:KD急性期T淋巴细胞亚群功能增强及血浆IL-10分泌上升说明机体免疫系统活化与免疫活性细胞的过度应答密切相关,血浆IL-10浓度的明显增高,可有效提高机体的免疫抗炎能力。
Objective: To investigate the relationship between plasma IL-10 and T cell subsets in children with Kawasaki disease (KD). Methods: Thirty children with KD were selected as the case group and 23 healthy children were randomly selected as the control group. The concentration of T-lymphocyte subsets and plasma IL-10 were determined by S-P single-stained method and ELISA double antibody sandwich method respectively. Results: The percentage of CD3 ~ + and CD4 ~ + cells in KD group increased in acute phase. But there was no significant difference between recovery and control group (P> 0.05); the percentage of CD8 ~ + cells was significantly decreased and CD4 ~ + / CD8 ~ + increased, which was significantly different from recovery and control groups (P <0.01). The concentration of plasma IL-10 in acute phase increased significantly, compared with the recovery phase, the control group had a significant difference (P <0.01). Coronary artery disease (CAL) group than the normal coronary artery (CA) in the acute phase of immune indicators more significant changes. Plasma IL-10 was negatively correlated with the percentage of CD8 + cells (r = -0.54, P <0.05). CONCLUSION: The increase of T lymphocyte subsets and the increase of plasma IL-10 secretion in the acute phase of KD suggest that the activation of the immune system is closely related to the excessive response of immunocompetent cells. The concentration of plasma IL-10 is significantly increased, which can effectively improve the immune response Inflammatory ability.