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目的:观察运动训练对大鼠局灶性脑梗死区周围皮质的胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)和生长相关蛋白-43(growth-associated protein,GAP-43)表达的影响。方法:成年健康雄性SD大鼠42只,随机分为3组:运动组,静止组,假手术对照组。运动组造模后给予电动跑台训练,每天30min;静止组造模后置于普通笼中饲养,不予康复训练;假手术对照组仅给予麻醉及头皮切开、缝合。采用光化学方法建立局灶性脑梗死模型,每组大鼠于术后7d,14d,21d处死,应用免疫组化方法观察各组大鼠不同时期梗死灶周围皮质GFAP和GAP-43的表达.结果:运动组梗死灶周围皮质GFAP的表达在14d和21d显著高于静止组和假手术对照组。运动组大鼠GAP-43表达在7d为最高,14d后开始下降,但均显著高于静止组和假手术对照组。静止组大鼠GAP-43表达在7d和14d高于假手术对照组。21d时三组大鼠GAP-43表达无明显差异。结论:运动训练能促进梗死灶周围皮质GFAP和GAP-43的表达,促进脑缺血后的突触发生与重建。
Objective: To observe the effect of exercise training on the expression of glial fibrillary acidic protein (GFAP) and growth-associated protein (GAP-43) in the surrounding cortex of rats with focal cerebral infarction. Methods: Forty-two healthy male SD rats were randomly divided into 3 groups: exercise group, rest group and sham operation control group. The rats in the exercise group were given electric treadmill training 30 minutes a day. The rats in the static group were housed in normal cages without rehabilitation training. The sham operation control group was given only anesthesia and scalp incision and suture. The model of focal cerebral infarction was established by photochemical method, and the rats in each group were sacrificed at 7d, 14d and 21d after operation.The expressions of GFAP and GAP-43 in the cortex around the infarct in different time groups were observed by immunohistochemical method.Results : The GFAP expression in the cortex around the infarct area in the exercise group was significantly higher than that in the static group and sham operation group on the 14th and 21st day. The expression of GAP-43 in exercise group rats was the highest at 7d, but decreased at 14d, but both of them were significantly higher than that of the rest and sham operation groups. The expression of GAP-43 in resting rats was higher than that in sham-operation control rats on 7d and 14d. There was no significant difference in GAP-43 expression between the three groups on 21d. Conclusion: Exercise training can promote the expression of GFAP and GAP-43 in the cortex around the infarct area and promote the synaptogenesis and remodeling after cerebral ischemia.