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目的:建立小承气汤中橙皮苷和肉桂酸的HPLC同时测定方法,以便进行质量控制。方法:采用Apollo C18柱(250mm×4.6mm,5μm),流动相为乙腈和0.011%冰乙酸水溶液,梯度洗脱,时间40min,流速1mL/min,检测波长为283nm。结果:橙皮苷和肉桂酸的线性范围分别为0.20-0.80μg和0.05-0.33μg,精密度为0.24%和0.16%,平均回收率分别为96.3%和101.9%。在6批样品中,橙皮苷和肉桂酸的含量差别比较大。结论:文章建立的方法简便、灵敏、准确,可用于小承气汤的质量控制。
Objective: To establish a HPLC simultaneous determination method for hesperidin and cinnamic acid in Xiao Cheng Qi Decoction for quality control. Methods: Apollo C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and 0.011% aqueous glacial acetic acid with a gradient of 40 min and a flow rate of 1 mL / min. The detection wavelength was 283 nm. Results: The linear ranges of hesperidin and cinnamic acid were 0.20-0.80μg and 0.05-0.33μg respectively, with the precision of 0.24% and 0.16%. The average recoveries were 96.3% and 101.9%, respectively. In six batches of samples, the content of hesperidin and cinnamic acid is quite different. Conclusion: The method established in this paper is simple, sensitive and accurate and can be used for the quality control of Xiao Cheng Qi Decoction.