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通过两种方法提取了烟草病叶中PVY病毒的RNA，然后针对PVY~N的CP基因序列，设计合成了一对特异性引物，运用反转录PCR技术对提取的PVY－RNA进行了体外扩增，结果得到与预期大小相一致的780bp片段，而对照未得到任何产物，从而建立了运用RT－PCR手段检测植物中PVY的又一有效途径，并运用此方法对东北三省部分地区马铃薯种薯中PVY的带毒情况进行了检测。
The RNA of PVY virus in diseased tobacco leaves was extracted by two methods. Then a pair of specific primers was designed and synthesized according to the CP gene sequence of PVY ~ N. The extracted PVY-RNA was amplified in vitro by reverse transcription PCR The result showed that the 780bp fragment was consistent with the expected size, but no product was obtained in the control. Thus, another effective way to detect PVY in plants by RT-PCR was established. By using this method, In PVY poisoning situation was tested.