论文部分内容阅读
目的:探讨不同剂量的甲泼尼龙(Methylprednisolone,MPS)对急性百草枯(paraquat,PQ)中毒大鼠早期肺损伤的疗效。方法:采用腹腔注射20%的PQ溶液制作大鼠急性PQ中毒的模型,随机均分为五组,正常对照组(A组)、染毒组(B组)、5mg/kg甲泼尼龙干预组(C组)、15mg/kg甲泼尼龙干预组(D组)、30mg/kg甲泼尼龙干预组(E组)。分三个不同时间点(24、72、168h)处死大鼠(每组每时间点6只)。观察各时间点大体标本,组织病理、肺系数和氧合指数。结果:光镜下肺组织病理学观察,与B组比较C、D、E组大鼠肺的病理学改变,肺泡腔内出血、渗出,炎性细胞浸润、肺泡隔炎性细胞浸润相对较轻,其中以C、E组减轻最为明显。在各组相同时间点肺系数:在三个时间点的值均比B组低(P<0.05),其中E组在24h、72h时间点上与C、D组有显著差异(P<0.05)。C、D、E组与B组的氧合指数的比较各个时间点上均与B组有差异(P<0.05),三组之间相互无明显差异。结论:本实验结果显示甲泼尼龙对急性百草枯中毒大鼠的肺损伤具有保护作用,且30mg/kg甲泼尼龙组要优于5mg/kg、15mg/kg甲泼尼龙组。
Objective: To investigate the effects of different doses of Methylprednisolone (MPS) on acute lung injury induced by acute paraquat (PQ) in rats. Methods: Acute PQ intoxication model was made by intraperitoneal injection of 20% PQ solution. The rats were randomly divided into five groups: normal control group (group A), exposure group (group B), 5 mg / kg methylprednisolone intervention group (Group C), 15 mg / kg methylprednisolone group (group D) and 30 mg / kg methylprednisolone group (group E). Rats were sacrificed at three different time points (24, 72, 168 h) (6 animals per group at each time point). Gross specimens, histopathology, lung coefficient and oxygenation index were observed at each time point. Results: The histopathological changes of lung in C, D and E groups were more obvious than those in B group. The pathological changes of lung, alveolar hemorrhage, exudation, infiltration of inflammatory cells, Which C, E group to reduce the most obvious. The lung coefficient at the same time point in each group was lower than that in group B at three time points (P <0.05), and the difference was significant between group E and C and D at 24h and 72h (P <0.05) . The oxygenation index of groups C, D, E and B were different from that of group B at all time points (P <0.05), and there was no significant difference among the three groups. CONCLUSION: The results of this experiment showed that methylprednisolone had a protective effect on lung injury in acute paraquat poisoning rats, and the methylprednisolone group at 30 mg / kg was superior to the methylprednisolone group at 5 mg / kg and 15 mg / kg.