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目的了解和掌握惠州市区腹泻患者沙门菌感染情况及菌型分布。方法采集定点医院就诊的腹泻患者粪便标本,采用传统方法与荧光定量PCR方法相结合检测沙门菌,用SPSS17.0软件对数据进行统计分析。结果 2472例腹泻患者中,检出沙门菌44例,阳性率为1.78%,共分离出7种血清型,其中鼠伤寒沙门菌26株,斯坦利沙门菌7株,肠炎沙门菌5株,雷根特沙门菌、德尔卑沙门菌各2株,乙型副伤寒沙门菌、阿贡纳沙门菌各1株。结论惠州市区腹泻患者主要以鼠伤寒沙门菌、斯坦利沙门菌和肠炎沙门菌感染引起的腹泻为主。通过加强对腹泻门诊腹泻患者沙门菌的监测,为防止食源性疾病的发生和控制起到良好的预警作用。
Objective To understand and master the Salmonella infection and the distribution of bacteria in diarrhea patients in Huizhou urban area. Methods Stool specimens from diarrhea patients in designated hospitals were collected. Salmonella was detected by a combination of traditional methods and real-time quantitative PCR, and the data were analyzed by SPSS17.0 software. Results Among 2472 patients with diarrhea, 44 cases were detected with positive rate of 1.78%. Seven serotypes were isolated, of which 26 were Salmonella typhimurium, 7 Salmonella Stanis, 5 Salmonella enteritidis and 5 were Salmonella enteritidis Two Salmonella typhi, two Salmonella delphi strains, one Salmonella paratyphi A strain and one Salmonella typhi strain. Conclusion The main diarrhea patients in Huizhou urban area are mainly caused by Salmonella typhimurium, Salmonella Stanley and Salmonella enteritidis. By strengthening the monitoring of Salmonella in patients with diarrhea outpatient diarrhea, to prevent the occurrence and control of foodborne diseases play a good early warning role.