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从杨树病斑上分离所得的水泡型溃疡病病原菌(Dothiorella gregariaSacc.),经室内外的人工接种试验,均能诱致发病,产生与自然罹病相同的典型症状。室内人工接种保湿时间以10～14天较为适宜,接种最适温度为24℃～28℃,温度低于13℃不发病。该菌对阔叶树中的柳树、刺槐、核桃均有致病性。不同杨树种及品种对该菌的抗病性测定结果,白杨派的种及品种较抗病,其中尤以新疆杨最抗病;青杨派、黑杨派×青杨派、青杨派×黑杨派的派间杂种最易感病,其中尤以青杨、小青杨、北京杨感病最重;黑杨派的品种为中等感病。
Dothiorella gregaria Saccharomyces cerevisiae isolated from the poplar lesion can induce the onset of the disease by in vitro and in vivo artificial inoculation tests with the same typical symptoms as the naturally occurring disease. Indoor artificial inoculation moisturizing time to 10 to 14 days is more appropriate, the optimal temperature for inoculation 24 ℃ ~ 28 ℃, the temperature is lower than 13 ℃ without disease. The bacteria on broad-leaved trees willow, black locust, walnut are pathogenic. Different poplar species and varieties of the disease resistance of the bacteria determination results, Populus species and varieties more resistant to disease, especially in Xinjiang Yang most resistant to disease; Qingyang school, Black Poplar sent Qingyang School, × Black Poppy sent the most susceptible to inter-party hybrids, especially in Qingyang, Xiaoqing, Beijing Yang most susceptible; Black Poppy is a moderate disease.