【摘 要】
：
研究优化了樟疫(Phytophthora cimamomi)多聚半乳糖醛酸酶Pcpg1、Pcpg2和Pcpg4基因克隆的PCR条件,并对其进行了克隆、测序和遗传转化.克隆获得了3个基因,其大小均为1 011bp;
【机 构】
：
西北农林科技大学园艺学院,Plant Cell Biology
论文部分内容阅读
研究优化了樟疫(Phytophthora cimamomi)多聚半乳糖醛酸酶Pcpg1、Pcpg2和Pcpg4基因克隆的PCR条件,并对其进行了克隆、测序和遗传转化.克隆获得了3个基因,其大小均为1 011bp;通过构建表达载体和遗传转化获得了3个基因的转基因菌系;3个基因均能指导合成相应的PG酶,其中转化Pcpg2.基因的酵母菌(Saccharomycescerevisiae)所分泌的多聚半乳糖醛酸酶(PG)酶活性最强,Pcpg4和对照Anpg(Aspergillusniger polygalacturonase)I次之,而Pcpg 1基因所指导合成的PG酶无活性.Western blotting表明,所克隆的3个基因所指导合成的PG酶均有不同程度的糖基化.
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