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目的:通过兔耳增生性瘢痕(HS)局部注射人参皂苷Rb_1,观察其治疗效果及探讨相关作用机制。方法:随机取8只新西兰大耳白兔,每耳6处创面,建立兔耳增生性瘢痕模型,上皮化后局部注射人参皂苷Rb_1,每3天1次,共3次。设空白组、地塞米松阳性对照组、生理盐水对照组、人参皂苷Rb_1组。注射后第1、2、4、8周切除标本行HE染色观察瘢痕增生指数(SEI),qPCR检测转化生长因子β(TGF-β)及Ⅰ型胶原表达。结果:注射后第4、8周,地塞米松及人参皂苷Rb_1组较生理盐水组HS瘢痕增生改善明显,瘢痕增生指数降低(P<0.05),TGF-β1及Ⅰ型胶原mRNA表达降低(P<0.05)。结论:人参皂苷Rb_1能有效改善兔耳增生性瘢痕,作用机制可能与抑制TGF-β1表达有关。
OBJECTIVE: To observe the therapeutic effect of Ginsenoside Rb_1 by injecting hypertrophic scars (HS) in rabbits ear and to explore its mechanism. Methods: Eight New Zealand white rabbits were randomly selected. Six rabbits per ear were used to establish the rabbit ear hypertrophic scars model. After epithelialization, ginsenoside Rb_1 was injected locally once every three days for 3 times. A blank group, dexamethasone positive control group, saline control group, ginsenoside Rb_1 group. At 1, 2, 4 and 8 weeks after the injection, the specimens were removed for HE staining to observe the scar proliferation index (SEI) and the expression of TGF-β and type Ⅰ collagen by qPCR. RESULTS: At 4 and 8 weeks after injection, dexamethasone and ginsenoside Rb_1 group had a significant improvement on HS scar proliferation, scar hyperplasia index (P <0.05) and TGF-β1 and type Ⅰ collagen mRNA (P <0.05). CONCLUSION: Ginsenoside Rb_1 can effectively improve the hypertrophic scars in rabbit ear. The mechanism may be related to the inhibition of TGF-β1 expression.