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为了解小麦在干旱逆境条件下的基因转录规律,采用PEG6000对耐旱小麦(Triticum aestivum)品种旱选10号进行拟旱处理,分别提取0、1、6和24h植株的总RNA,经反转录荧光标记制备cDNA探针,并将其与含有6万Oligo的水稻全基因组芯片进行杂交,扫描采集数据后并进行结果分析。在1、6和24h样品中分别检测到差异表达基因166、207和328个,随着处理时间的延长,差异表达基因数目增加。对差异基因进行功能分类,能量代谢途径相关基因在1、6和24h差异表达基因总数中所占比例分别为4.2%、8.2%和16.8%,其中大部分为光合作用相关基因,并且主要表现为上调,但Psbr和Rubisco编码基因的转录水平为下调,暗示它们在耐旱反应中发挥着一定作用。
In order to understand the gene transcriptional regulation of wheat under drought stress conditions, PEG6000 was used to treat drought-tolerant wheat (Triticum aestivum) Variety Xuanxuan No.10, and total RNA was extracted from plants at 0, 1, 6 and 24 h respectively. Fluorescently labeled cDNA probes were prepared and hybridized with a genome-wide rice genome containing 60,000 Oligo. The collected data were scanned and analyzed. A total of 166, 207 and 328 differentially expressed genes were detected in the samples at 1, 6 and 24 h, respectively. The number of differentially expressed genes increased with the prolongation of treatment time. The functional classification of differential genes, energy metabolism pathway-related genes in the total number of differentially expressed genes at 1, 6 and 24h were 4.2%, 8.2% and 16.8%, most of them are photosynthesis-related genes, and mainly as But the transcriptional levels of Psbr and Rubisco encode genes are downregulated, suggesting that they play a role in the drought tolerance response.