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选择我国棉花优良品种泗棉３号进行组织培养，获得了具有高频胚胎发生的愈伤组织、胚状体和小植株。在附加ＺＴ的ＭＳ培养基上，泗棉３号的下胚轴、子叶、根均诱导获得了愈伤组织，但其形成率和形成量不同，其中下胚轴较易诱导获得愈伤组织，根和子叶较差。ＺＴ对愈伤组织形成和生长形响较大，其在愈伤组织诱导中较适宜的使用浓度为３．０或５．０ｍｇ／Ｌ。诱导获得的愈伤组织不经继代培养，７０天后可形成两类性质不同的愈伤组织：胚性愈伤组织和非胚性愈伤组织。将胚性愈伤组织转入不含任何激素的培养基上，２０天后可形成不同发育时期的体细胞胚。不同外植体体细胞发生的能力不同，根较易，胚轴次之，子叶较差。低浓度的ＺＴ有利于胚性愈伤组织的产生。不同培养基对泗棉３号胚性愈伤组织增殖、体细胞胚形成和萌发的影响不同。２，４－Ｄ有利于胚性愈伤组织的增殖，但不利于体细胞胚形成和萌发；活性炭的添加或ＺＴ与ＩＡＡ的合理配合可促进体细胞胚形成和萌发。木试验中最有利于胚性愈伤组织增殖的培养基为ＺＨ＋１．０ｍｇ／Ｌ２，４－Ｄ＋０．５ｍｇ／ＬＫＴ＋０．５ｍｇ／Ｌ ＺＴ；最有利于体细胞胚形成和萌发的培养基为ＭＳ＋Ｂ＿５＋０．１ｍｇ／ＬＺＴ＋２ｇ／Ｌ活性炭。胚性愈伤组织不断在?
The cotton cultivar Simian No.3 in China was selected for tissue culture, and callus, embryoid body and plantlet with high frequency embryogenesis were obtained. On the MS medium supplemented with ZT, the callus was induced in hypocotyls, cotyledons and roots of Simian No.3, but its formation rate and amount were different. The hypocotyls were easier to induce callus, Roots and cotyledons are poor. ZT had a great influence on callus formation and growth, and its suitable concentration for callus induction was 3.0 or 5.0 mg / L. The induced callus without subculture, 70 days after the formation of two different types of callus: embryogenic callus and non-embryogenic callus. Embryogenic calli were transferred to medium without any hormones, and somatic embryos of different developmental stages could be formed after 20 days. The ability of different explants to produce somatic cells is different, the roots are easier, the hypocotyls are second, and the cotyledons are poorer. Low concentrations of ZT favor embryogenic callus. The effects of different media on the proliferation, somatic embryogenesis and germination of Simian 3 embryogenic callus were different. 2,4-D is conducive to the proliferation of embryogenic callus, but not conducive to the formation and germination of somatic embryos; the addition of activated carbon or ZT with IAA can promote the formation and germination of somatic embryos. The best culture medium for embryogenic callus in wood experiment was ZH + 1.0mg / L 2,4-D + 0.5mg / L KT + 0.5mg / L ZT. The medium most conducive to somatic embryogenesis and germination was MS + B_5 + 0. 1mg / LZT + 2g / L activated carbon. Embryogenic calluses constantly?