Background Recurrence and local lymph node metastasis affected the prognosis of patients with laryngeal cancer.The aim of this study was to analyze clinical and pathological significance of vasculogenic mimicry (VM) in laryngeal squamous cell carcinoma (LSCC) and evaluate its contribution to prognosis.Methods Data of 168 cases of LSCC were reviewed retrospectively to reveal clinical pathology and prognostic significance of VM.CD31 and periodic acid-Schiff double staining was used to identify VM.Results VM in LSCC contributed to lymph node metastasis (P=0.003) and clinical progression.VM correlated to histopathology grade (P=0.001) of LSCC.VM was an adverse prognostic factor for both disease-specific survival (P=0.039) and metastasis-free survival (P=0.042) by univariate survival analyses.And it was an independent prognostic factor for only disease-specific survival (P=0.003) by multivariate survival analyses.Conclusions VM existed in LSCC.LSCC with VM has more potential to invasion and metastasis.