论文部分内容阅读
本研究先分别采用酸热法和酶法对红发夫酵母菌体进行破壁处理,然后对二者共存时的破壁条件进行了正交优化。结果表明,在60℃用2mol/L的盐酸处理6h效果最好,类胡萝卜素提取率达到85.32%,对虾青素的破坏率最小;用7mL溶菌酶在50℃处理6h效果最好,类胡萝卜素提取率达到78.43%,对虾青素几乎没有破坏作用。酸和酶共存时破壁条件的优化结果表明,在50℃用2mol/L的盐酸和9mL的溶菌酶处理4h时类胡萝卜素提取率最高,达到95.62%,而且反应条件更温和、处理时间缩短、对类胡萝卜素破坏作用减小。在破壁后、菌体干燥前,用NaOH中和反应液中的盐酸使得终点pH值为3.0时效果最好,类胡萝卜素提取率提高到99.23%,表明经过处理的红发夫酵母菌体可以用作饲料添加剂。
In this study, the acid-heat method and enzymatic method were used to break down the Phaffia rhodozyma cells, respectively, and then the orthogonal conditions were optimized for the conditions of the two strains. The results showed that the treatment with 2mol / L hydrochloric acid at 60 ℃ for 6h had the best effect, the extraction rate of carotenoids reached 85.32%, and the destroying rate of astaxanthin was the lowest. Treating 7h with lysozyme at 50 ℃ for 6h was the best, Su-extraction rate of 78.43%, almost no damage to astaxanthin. The optimal conditions of acid and enzyme coexistence of the broken wall showed that the extraction rate of carotenoids was the highest at 95.62% when treated with 2mol / L hydrochloric acid and 9mL of lysozyme at 50 ℃ for 4h, and the reaction conditions were more mild and the treatment time was shortened , The destruction of carotenoids reduced. After the broken wall, before the bacterial cells were dried, NaOH was added to neutralize the reaction solution to make the best pH value of 3.0, and the extraction rate of carotenoids increased to 99.23%, indicating that treated Phaffia rhodozyma Can be used as feed additives.