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目的 探讨Bc1 2和Bax基因在病毒性心肌炎进展中的作用。方法 柯萨奇病毒B3 (CVB3 )感染12 5只雄性Balb/c小鼠 ,分别于d7、10、14、2 1、2 8随机取 2 0只实验组小鼠和 5只对照组小鼠处死 ,采用免疫组织化学方法检测CVB3 病毒性心肌炎小鼠不同时期心肌组织中Bcl 2和Bax蛋白表达情况。结果 病毒性心肌炎发病率为 86 % ,其中 80 %小鼠为轻、中度病变。正常对照组心肌中 ,无Bc1 2蛋白表达。在实验组中Bc1 2蛋白表达的动态变化与心肌病变积分呈显著正相关 (r =0 .93 P <0 .0 1) ,感染后 7～ 14d ,实验组小鼠心肌组织中Bax蛋白表达显著高于正常对照组 (P <0 .0 5 )。少数心肌浸润淋巴细胞表达Bc1 2和Bax。结论 Bc1 2和Bax基因参与病毒性心肌炎细胞凋亡的调控。
Objective To investigate the role of Bc1 2 and Bax genes in the progression of viral myocarditis. Methods Coxsackievirus B3 (CVB3) was used to infect 12 5 male Balb / c mice, and 20 mice in experimental group and 5 control group were randomly assigned to die on days 7, 10, 14, Immunohistochemistry was used to detect the protein expression of Bcl 2 and Bax in myocardium of CVB3 myocarditis mice at different stages. Results The incidence of viral myocarditis was 86%, of which 80% were mild to moderate. In the normal control group, no Bc1 2 protein was expressed. In the experimental group, the dynamic changes of Bc1 2 protein expression were positively correlated with the scores of myocardial lesion (r = 0.93, P <0.01). From 7 to 14 days after infection, the expression of Bax in the myocardium of experimental mice was significantly increased Higher than the normal control group (P <0.05). A few myocardial infiltrating lymphocytes express Bc1 2 and Bax. Conclusion Bc1 2 and Bax genes are involved in the regulation of apoptosis in viral myocarditis.