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肥大细胞广泛分布于各个组织,它与多种人类疾病的发病机理有关。肥大细胞的异质性明显与生理、病理生理和治疗有关。作者取不同皮肤肿瘤手术切除后的正常皮肤标本37份,其中24份良性皮损,13份恶性皮损进行研究。所有标本等分为2,一半用10%中性福尔马林固定,另一半用 Carnoy 氏溶液固定,石蜡包埋、切片,并用甲苯胺蓝,阿辛蓝/番红和 HE3种方法染色。观察到真皮肥大细胞颗粒着色有3种类型:肥大细胞的绝大多数只含有阿辛蓝阳性颗粒(蓝色);少数只含有番红阳性颗粒(红色);或含有阿辛蓝阳性和番红阳性混合颗粒。肥大细胞用阿辛蓝染色比用甲苯胺蓝染色数量明显增加(P<0.001),Carnoy 氏固定液固定正常组织,用阿辛蓝/番红染色比甲苯胺蓝染色着色的肥大细胞多达2
Mast cells are widely distributed in various tissues and are involved in the pathogenesis of various human diseases. The heterogeneity of mast cells is obviously related to physiology, pathophysiology and treatment. The authors took 37 normal skin specimens after surgical resection of different skin tumors, including 24 benign lesions and 13 malignant lesions. All specimens were equally divided into 2, half were fixed in 10% neutral formalin and the other half were fixed in Carnoy’s solution, paraffin-embedded, sectioned, and stained with toluidine blue, Alcian blue/carcinogen and HE3 methods. There are three types of granulocyte coloring of dermal mast cells: the vast majority of mast cells contain only Alcian blue-positive particles (blue); a few contain only safranine-positive particles (red); or contain Alcian blue-positive and safranin. Positive mixed particles. Mast cells stained with Alcian blue stained with toluidine blue significantly increased (P<0.001), Carnoy’s fixative fixated normal tissue, and stained with Alcian blue/scarlet stained toluidine blue stained mast cells up to 2