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以“豫楸1号”为试材,研究了灭菌方法、基本培养基类型、激素浓度和取材时间对“豫楸1号”茎段腋芽诱导的影响。结果表明:不同基本培养基对“豫楸1号”腋芽诱导的影响差异显著,MS为基本培养基诱导腋芽的诱导率最高;多菌灵浊液浸泡60min、酒精灭菌30s、升汞浸泡9min的灭菌方法最适合“豫楸1号”诱导腋芽萌发;“豫楸1号”最适的腋芽诱导培养基为MS+0.8mg/L 6-BA+0.10mg/L IBA;建立“豫楸1号”组培快繁体系最佳取材时间是4月,染菌率、褐化率低,腋芽诱导率高,腋芽萌发、生长快。
The effects of sterilization method, basic medium type, hormone concentration and time on the induction of axillary buds in the stem of “Yu-Catalpa 1” were studied using “Yu-Catalpa 1” as test material. The results showed that the effects of different basic media on the induction of axillary buds were significant, and the induction rate of axillary buds was the highest with MS as basal medium. After soaking in carbendazim turbid solution for 60min, alcohol sterilization for 30s and mercuric chloride The most suitable axillary bud induction medium was “MS + 0.8mg / L 6-BA + 0.10mg / L” IBA; establishment “” Yu Catalpa 1 "tissue culture fast propagation system is best drawn in April, the rate of infection, browning rate is low, induction rate of axillary buds, axillary buds germination, rapid growth.