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目的 :验证性腺激素对瘦素分泌的影响 ,寻找妊娠期瘦素峰出现的原因。方法 :用 3月龄的金黄仓鼠作为实验动物。制备雌激素及孕激素硅胶埋植管、检验其释放剂量以用于提供后续实验中持续性的高水平雌激素及孕酮 ;制备雌激素以及抑制素的抗血清、检验其生物活性 ,用于后续实验中消减体内这两种激素的生物学作用 ,观察这两种激素缺失对于瘦素分泌活动的影响 ;通过妊娠期间卵巢切除来观察该期间内性腺对于瘦素分泌活动的影响。通过对于切除性腺的动物激素埋植的方法来观察不同激素对在体时瘦素分泌的作用。结果 :金黄仓鼠于妊娠第 11天去除卵巢后瘦素水平显著下降 ,妊娠第 12天的瘦素峰消失 ;妊娠第 11天注射雌激素及抑制素抗血清对瘦素的分泌没有产生显著影响 ;与正常对照相比 ,未孕的金黄仓鼠切除卵巢后瘦素水平显著下降 (p <0 0 5 ) ,切除卵巢后单独埋植雌激素、孕酮不能阻止瘦素水平的下降 ,但是当埋植孕酮和雌激素复合物时瘦素的水平得到恢复。这些结果说明性腺激素对于瘦素的分泌有重要的调节作用。单独的雌激素或孕激素对瘦素分泌没有影响 ,但雌激素与孕激素复合物却对于瘦素的分泌有重要的调节作用。结论 :妊娠后期高水平的雌激素、孕激素等性腺激素的综合作用可能促成了该期瘦素水平的上升。
Objective: To verify the effect of gonadal hormones on the secretion of leptin and to find out the causes of leptin peak during pregnancy. Methods: Three-month-old golden hamsters were used as experimental animals. Estrogen and progestin silicone implant tubes were prepared and their released dose was tested for sustained high levels of estrogen and progesterone in subsequent experiments; antisera to estrogen and inhibin were prepared and tested for bioactivity for Follow-up experiments to reduce the biological effects of these two hormones in vivo to observe the impact of these two hormones on leptin secretion; ovarian resection during pregnancy to observe the effect of gonadal secretion of leptin during the period. The effect of different hormones on the secretion of leptin in vivo was observed by implanting animal hormones for resection of the gonads. Results: After the golden hamster was ovariectomized on the 11th day of gestation, the leptin level decreased significantly and the leptin peak disappeared on the 12th day of gestation. The estrogen and inhibin antisera did not significantly affect the leptin secretion on the 11th day of gestation. Compared with the control, the level of leptin in ovariectomized golden hamsters was significantly decreased (p <0.05). After ovariectomized, estrogen and progesterone could not prevent the decrease of leptin levels. However, The level of leptin recovered during the progesterone and estrogen complex. These results indicate that gonadal hormones play an important regulatory role in the secretion of leptin. Estrogen or progestin alone has no effect on leptin secretion, but estrogen and progestin complexes have an important regulatory role for the secretion of leptin. Conclusion: The combined effects of high levels of estrogen, progesterone and other gonadal hormones during late gestation may lead to the increase of leptin level in this period.