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利用竹红菌甲素并结合其光敏特性进行了动物体内的抑瘤试验。发现患瘤局部表皮涂布竹红菌甲素软膏或局部皮下注射或腹腔注射甲素花生油溶液、并经光照致敏后能使小鼠S-180实体肉瘤生长减缓,甚至有个别消退。同时利用细胞培养法和~3H标记化合物参入法进行了甲素抑瘤作用的定量分析。发现作用时间固定时(光照40分钟后继续培养3小时),大于12.5ng/ml浓度的甲素能明显抑制~3H-TdR、~3H-UR和~3H-Leu对S-180肉瘤细胞的参入速率;剂量与抑制率呈正相关。据此结果求出甲素对S-180肉瘤细胞的有效抑制浓度(LD(50))为40ng/ml。光照组与无光照组差异有显著性。用Ebrlich腹水癌细胞为研究体系也得到了类似的试验结果。实验结果提示竹红菌甲素具有明显的抑瘤作用,这种作用与其光敏特性有关。
Utilizing the hypocrellin A combined with its photosensitizing properties, a tumor-inhibition test was performed in animals. It was found that the local epidermal epidermis was coated with hypocrellin A or the subcutaneous injection or intraperitoneal injection of Ala peanut oil solution, and after sensitization by light, the growth of S-180 solid sarcoma in mice was slowed down and even subsided. At the same time, quantitative analysis of anti-tumor effect of A was performed using cell culture method and ~3H-labeled compound incorporation method. When the time of action was fixed (3 hours after light irradiation for 40 minutes), A larger than 12.5 ng/ml significantly inhibited the incorporation of ~3H-TdR, ~3H-UR, and ~3H-Leu into S-180 sarcoma cells. Rate; dose and inhibition rate was positively correlated. Based on this result, it was found that the effective inhibitory concentration (LD(50)) of A-S on S-180 sarcoma cells was 40 ng/ml. There was a significant difference between the light and non-light groups. Ebrlich ascites cancer cells were used as the research system and similar results were obtained. The experimental results suggest that hypocrellin A has a significant anti-tumor effect, and this effect is related to its photosensitive properties.