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通过多代淘汰的方法,筛选了一组稳定高效的棉秆降解复合菌系-MEG复合菌系。将该菌系接入棉秆,静置处理7d后进行沼气发酵,其产气量较未经预处理的提高了25%。变性梯度凝胶电泳(DGGE)分析结果表明,生物预处理后棉秆沼气发酵系统的细菌群落结构多样性较未经预处理的丰富。同时,比较了采用6种驯化方法获得的接种物对棉秆沼气发酵日产气量和累积产气量的影响,结果表明,采用河底泥、臭水沟泥、工厂废水底泥、荷塘底泥、湖底泥等5种污泥混合后驯化的活性污泥可作为棉秆沼气发酵的有效接种物,其沼气发酵的日产气量和累积产气量均较单种污泥驯化的接种物高。
Through a series of elimination methods, a group of stable and efficient cotton straw degradation composite strain-MEG complex strains were screened. The strain was connected to cotton stalks, biogas fermentation was carried out after standing for 7 days, and its gas production was increased by 25% compared with that without pretreatment. The results of denaturing gradient gel electrophoresis (DGGE) showed that the bacterial community structure diversity of cotton stalk biogas fermentation system after biological pretreatment was more abundant than that without pretreatment. At the same time, the effects of inoculants obtained by six domestication methods on the daily gas production and cumulative gas production of cotton stalk biogas fermentation were compared. The results showed that the use of river bottom sludge, stinky gully mud, factory wastewater sediment, Activated sludge mixed with 5 kinds of sludge, such as mud, can be used as an effective inoculum for cotton stalk biogas fermentation. The daily gas production and cumulative gas production of biogas fermentation are higher than those of domesticated inoculum of single sludge.