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目的:观察一枝黄花煎剂及其提取物的抗菌试验效果。方法:采用微量琼脂稀释法在体外分别测定一枝黄花煎剂及其提取物(总皂苷、总黄酮)对革兰阳性菌如金黄色葡萄球菌(Sa)标准菌株及Sa临床菌株、枯草杆菌和革兰阴性菌如大肠埃希菌、福氏志贺菌、伤寒沙门菌、奇异变形杆菌和铜绿假单胞菌标准菌株的抗菌效应。结果:一枝黄花总皂苷(浓度≤16mg/ml)对试验菌均未显示出抑菌作用;一枝黄花煎剂对金黄色葡萄球菌有较强的抑制作用,但对其他试验菌株的抑制作用很弱(MIC≥100mg/ml生药浓度);一枝黄花总黄酮对试验菌株均有不同程度的抗菌活性(MIC范围为0.25mg/ml~4.0mg/ml)。一枝黄花煎剂、一枝黄花总黄酮对Sa标准菌株的最低抑菌浓度(MIC)分别为12.5mg/ml和0.5mg/ml;对15株Sa临床菌株的MIC范围分别为6.25mg/ml~50.0mg/ml和0.25mg/ml~4.0mg/ml,MIC均数分别为22.083mg/ml和1.283mg/ml,MIC50分别为12.5mg/ml和1.0mg/ml,MIC90分别为50.0mg/ml和2.0mg/ml,经统计学处理两者差异有显著性(t=5.252,P<0.01)。结论:一枝黄花具有较强的抗菌作用,不同提取方法的抗菌效果存在一定的差异,以黄酮类化合物抗菌效果最好,且对革兰阳性菌的抑菌作用比对革兰阴性菌的抑菌作用要强。
OBJECTIVE: To observe the antibacterial effect of a yellow flower decoction and its extract. METHODS: Astragalus decoction and its extracts (total saponins, total flavonoids) against gram-positive bacteria such as the standard strain of Staphylococcus aureus (Sa) and Sa clinical strain, Bacillus subtilis, and leather were determined in vitro using a trace agar dilution method. Gram-negative bacteria such as Escherichia coli, Shigella flexneri, Salmonella typhi, Proteus mirabilis, and Pseudomonas aeruginosa have antibacterial effects. RESULTS: The total saponins of saponins (concentration ≤ 16 mg/ml) did not show antibacterial activity against the test bacteria; S. frondosa had a strong inhibitory effect on Staphylococcus aureus, but the inhibitory effect on other test strains was weak. (MIC ≥ 100 mg/ml crude drug concentration); total flavonoids of Solidago sinensis has different degrees of antibacterial activity against the test strain (MIC range is 0.25mg/ml~4.0mg/ml). The minimal inhibitory concentration (MIC) of total flavonoids from Solidago canadensis and Safflower was respectively 12.5 mg/ml and 0.5 mg/ml for Sa standard strains, and 6.25 mg/ml~50.0 for 15 Sa clinical strains, respectively. Mg/ml and 0.25 mg/ml to 4.0 mg/ml, the mean MICs were 22.083 mg/ml and 1.283 mg/ml, the MIC50 were 12.5 mg/ml and 1.0 mg/ml, and the MIC90 was 50.0 mg/ml respectively. 2.0mg/ml, statistically significant difference between the two (t = 5.252, P <0.01). Conclusion: Solidago canadensis has a strong antibacterial effect. There are some differences in the antibacterial effects of different extraction methods. The antibacterial effect of flavonoids is best, and the antibacterial effect against Gram-positive bacteria is better than that against Gram-negative bacteria. Stronger role.