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目的探索淫羊藿素对人脐带间充质干细胞(UC-MSC)体外增殖及细胞因子分泌的影响。方法分离、培养人脐带来源的间充质干细胞(MSC),采用含不同浓度淫羊藿素的培养基培养,流式细胞术检测MSC表面标志物,Brd U法检测MSC的增殖能力,实时荧光定量PCR法检测MSC分泌细胞生长因子的变化。结果不同浓度(10,20,50,100 ng·mL~(-1))的淫羊藿素均可促进MSC增殖(P<0.05),其中以20,50 ng·mL~(-1)组促增殖作用最明显;与对照组比较,淫羊藿素对尿激酶纤溶酶原激活物(u PA,10 ng·mL-)1、基质金属蛋白酶-1(MMP-1,10,20,50 ng·mL-)1、血管内皮生长因子(VEGF,50 ng·mL~(-1))mR NA的表达明显增加,差异有统计学意义(P<0.05)。结论淫羊藿素可促进UC-MSC体外增殖,并且促进肝细胞生长因子(HGF)、u PA、MMP-1、VEGF的表达。
Objective To explore the effect of icaritin on the proliferation and cytokine secretion of human umbilical cord mesenchymal stem cells (UC-MSCs) in vitro. Methods Human umbilical cord-derived mesenchymal stem cells (MSCs) were isolated and cultured. Cultured with different concentrations of icaritin, the surface markers of MSC were detected by flow cytometry. The proliferation of MSC was detected by BrdU assay. Real-time fluorescence Quantitative PCR was used to detect the changes of secreted cell growth factor in MSC. Results Icaritin at different concentrations (10, 20, 50, 100 ng · mL -1) promoted the proliferation of MSC (P <0.05) The effect of Icaritin on urokinase plasminogen activator (uPA, 10 ng · mL-) 1, matrix metalloproteinase-1 (MMP-1, 10, 20, 50 ng · ML-) 1, the expression of vascular endothelial growth factor (VEGF, 50 ng · mL -1) mR NA was significantly increased, the difference was statistically significant (P <0.05). Conclusion Icaritin can promote the proliferation of UC-MSC in vitro and promote the expression of hepatocyte growth factor (HGF), u PA, MMP-1 and VEGF.