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采用分离自健康茶树叶片组织的一株长枝木霉Trichoderma longibrachiatum菌株CSN-18,研究茶树内生木霉的人工接种、再分离及其在茶树地上部组织中的内生性。该菌在PDA培养基上生长速度快,产孢量大。用CSN-18回接茶苗,接种后一个月可以从茶苗的茎和叶组织中再分离获得该真菌。接种后的茶苗没有观察到明显的病害表现;与对照相比,接种后6个月组培苗生长良好,叶色更绿;接种后1个月,实生苗生长正常。通过石蜡切片和苯胺蓝染色,在已接种的茶树组培苗的叶片内部组织中可观察到木霉的存在,从而证明了木霉能够在茶树地上部组织内定殖,是茶树的一种内生真菌。
A Trichoderma longibrachiatum strain CSN-18 isolated from the leaves of healthy tea plants was used to study the artificial inoculation and reisolation of endophytic Trichoderma from tea plants and their endophytic activity in the aerial part of tea plant. The bacteria grew fast on PDA medium with large amount of sporulation. The calli were picked back with CSN-18 and one more month after inoculation, the fungus was isolated from the stem and leaf tissue of the tea shoots. After inoculated tea seedlings, no obvious disease symptoms were observed. Compared with the control, the tissue culture seedlings grew well and the leaf color was greener at 6 months after inoculation. The seedlings grew normally one month after inoculation. Through the paraffin section and aniline blue staining, the presence of Trichoderma was observed in the internal tissues of the inoculated tea plantlets, demonstrating that Trichoderma can colonize in the upper tissues of the tea plant and is an endophyte of the tea plant Fungus.