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目的:建立以HPLC法测定阴炎净洗液中苦参碱的方法。方法:色谱柱为Lichrospher NH_2(250mm×4.6mm,5μm);流动相为乙腈-无水乙醇-3%磷酸(80:10:10);流速为1.2ml·min~(-1);柱温为室温;检测波长为208nm。结果:苦参碱在14～560μg·ml~(-1)浓度范围内与峰面积积分值呈良好线性关系(r=0.999 9),平均回收率为97.0%,RSD=0.7%(n=6)。结论:该方法简便、快速、准确,可用于该制剂的质量控制。
Objective: To establish a HPLC method for the determination of matrine in Yinyanxin lotion. Methods: The chromatographic column was Lichrospher NH 2 (250mm×4.6mm, 5μm); the mobile phase was acetonitrile-anhydrous ethanol-3% phosphoric acid (80:10:10); the flow rate was 1.2ml·min-1; Room temperature; detection wavelength is 208 nm. Results: Within the range of 14-560 μg·ml -1 , matrine had a good linear relationship with the integral value of the peak area (r=0.999 9). The average recovery was 97.0% and RSD was 0.7% (n=6). ). Conclusion: This method is simple, rapid and accurate and can be used for the quality control of the preparation.