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目的探讨Ig基因重排检测在B细胞性淋巴瘤中的诊断价值。方法选取B细胞性淋巴瘤30例、淋巴组织反应性增生30例,提取DNA,应用BIOMED-2引物系统中的47条引物进行PCR扩增,核酸分子异源双链凝胶电泳分析Ig基因重排结果。结果 30例B细胞性淋巴瘤中检测出Ig克隆性重排,包括IGH(A+B)克隆性重排23例,IGK克隆性重排23例,IGL克隆性重排5例,IGH(A+B)+IGK克隆性重排30例,IGHA+IGK克隆性重排29例;在30例淋巴组织反应性增生中未检测到Ig克隆性重排。结论 Ig基因重排是诊断B细胞性淋巴瘤的有用工具。
Objective To investigate the diagnostic value of Ig gene rearrangement in B cell lymphoma. Methods Thirty cases of B-cell lymphoma and 30 cases of lymphoid tissue hyperplasia were selected for DNA extraction. 47 primers in BIOMED-2 primer system were used for PCR amplification. The results of heterologous double-stranded gel electrophoresis Row results. RESULTS: Clonal Ig rearrangements were detected in 30 cases of B cell lymphoma, including 23 cases of clonal rearrangement of IGH (A + B), 23 cases of IGK clonal rearrangement, 5 cases of IGL clonal rearrangement, 1 case of IGH (A + B) + IGK clonal rearrangement in 30 cases, IGHA + IGK clonal rearrangement in 29 cases; no Ig clonal rearrangement was detected in 30 cases of lymphoid reactive hyperplasia. Conclusion Ig gene rearrangement is a useful tool to diagnose B-cell lymphoma.