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目的 :观察丙泊酚、咪达唑仑和硫喷妥钠对大鼠局灶脑缺血再灌注后的神经功能缺陷和病理学后果的影响。方法 :雄性SD大鼠 ,采用大脑中动脉内线栓法 (MCAO)诱发局灶脑缺血。经历 3h缺血和 2 4h再灌注后 ,按 0～ 5分级标准判断神经功能缺陷程度。经氯化三苯四唑 (TTC)染色后 ,用Im age分析系统计算脑梗死容积 ,电镜下观察梗死边缘组织的细胞超微结构。结果 :丙泊酚和咪达唑仑改善大鼠神经功能缺陷程度 ,降低脑梗死和脑水肿容积 ,并减少组织超微结构损伤。且丙泊酚的保护作用强于咪达唑仑。硫喷妥钠未显示脑保护作用。结论 :丙泊酚和咪达唑仑有拮抗大鼠局灶脑缺血后再灌注损伤的作用
Objective: To observe the effects of propofol, midazolam and thiopental on neurological deficits and pathological consequences after focal cerebral ischemia-reperfusion in rats. Methods: Male Sprague Dawley rats were induced focal cerebral ischemia by middle cerebral artery occlusion (MCAO). After 3h ischemia and 24h reperfusion, according to 0 ~ 5 grading standards to determine the degree of neurological deficit. After staining with triphenyltetrazolium chloride (TTC), the volume of cerebral infarction was calculated by Im age analysis system, and the ultrastructure of the infarct tissue was observed under electron microscope. Results: Propofol and midazolam improved the degree of neurological deficit, decreased the volume of cerebral infarction and cerebral edema, and reduced the ultrastructure damage. And the protective effect of propofol is stronger than midazolam. Thiopental does not show brain protection. CONCLUSION: Propofol and midazolam have antagonistic effects on reperfusion injury after focal cerebral ischemia in rats