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将人肺腺癌细胞ＳＰＣ、Ａ５４９、Ａ４２７序贯免疫ＢＡＬＢ／Ｃ小鼠，取脾细胞与小鼠骨髓瘤细胞ＳＰ２／０融合。获得一株稳定分泌抗肺腺鳞癌单克隆抗体的杂交瘤细胞株──Ｓ５Ａ１０─２，制备了小鼠腹水抗体并进行了纯化，腹水效价为１．０２×１０７，亚型测定为ＩｇＧ１．免疫组化分析ｓ５Ａ１０－２单抗与肺腺、鳞癌和肺鳞癌有较好的选择反应性，与正常组织无交叉反应，ＷｅｓｔｅｒｎＢｌｏｔ测定Ｓ５Ａｌ０─２在ＳＰＣ细胞膜上的抗原分子量为４０Ｋｄ、５０Ｋｄ和８０Ｋｄ，在Ａ５４９，细胞膜上的抗原分子量为４３Ｋｄ，而与Ａ４２７细胞膜蛋白无明显反应。该抗体的亲和常数为８．４３×１０１０Ｍ－１，Ｓ５Ａ１０－２杂交瘤细胞株，经液氮冻存后复苏，其分泌抗体能力不受影响。
BALB / C mice were immunized with human lung adenocarcinoma cell line SPC, A549 and A427 sequentially. The spleen cells were fused with mouse myeloma cells SP2 / 0. Obtained a stable hybridoma cell line secreting anti-lung adenosquamous carcinoma monoclonal antibody ─ ─ S5A10 ─ 2, mouse ascites antibody was prepared and purified ascites titer of 1.02 × 107, the subtype was determined as IgG1 . Immunohistochemical analysis of s5A10-2 mAb with lung adenocarcinoma, squamous cell carcinoma and lung squamous cell carcinoma have a good choice of response, and no cross-reaction with normal tissue, WesternBlot determination of S5Al0 ─ 2 SPC membrane antigen molecular weight of 40Kd, 50Kd And 80Kd, in A549, the molecular weight of the antigen on the cell membrane was 43Kd, but no significant reaction with the A427 cell membrane protein. The antibody affinity constant of 8.43 × 1010M-1, S5A10-2 hybridoma cell line, after liquid nitrogen recovery after cryopreservation, its ability to secrete antibodies are not affected.