ｐ16基因在恶性胶质瘤仅纯合缺失就高达 3 3 %～ 85 % ,在胶质瘤的发生发展中无疑起着重要作用。将ｐ16基因转染胶质瘤细胞系可起明显抑制作用 ,提示ｐ16基因将在胶质瘤的基因治疗中有美好的前景。但对肿瘤细胞转染ｐ16基因后对化疗药物是否产生耐药存在争议。本研究用ｐ16基因 The loss of p16 gene in homozygous malignant gliomas is as high as 3% to 85%, which undoubtedly plays an important role in the development of gliomas. The p16 gene transfection of glioma cell lines can be significantly inhibited, suggesting that p16 gene will have a bright future in gene therapy of glioma. However, there is controversy as to whether chemotherapeutic drugs are resistant to tumor cells transfected with p16 gene. The p16 gene was used in this study.