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目的研究宫内和出生后慢性缺氧对子代大鼠血清血管紧张素Ⅱ(AngⅡ)及脂联素(ADP)的影响。方法清洁级SD雌性孕7 d大鼠20只随机分为慢性间断性缺氧组(I)和常氧组(N);子代鼠出生后3周龄各组选取雄性子代鼠再次随机分为常氧组(C)和慢性间断性缺氧组(H);缺氧组按照慢性间断性缺氧大鼠模型给予缺氧处理,每日缺氧8 h,孕鼠从孕7 d直至分娩,子代大鼠自出生后持续处理12周。常氧组常规饲养。实验末,采集子代大鼠血清,用ELISA法测血清血管紧张素Ⅱ(AngⅡ),夹心法酶联免疫吸附法测定血浆脂联素(ADP)。结果子代大鼠给予常氧时,宫内缺氧子代大鼠血清AngⅡ水平显著高于宫内给予常氧的子代大鼠(2.39±0.45 pg/ml vs.2.06±0.12 pg/ml,P<0.05);而ADP水平显著低于后者(12.03±1.14 mg/L vs.14.15±1.72 mg/L,P<0.05)。当子代大鼠给予缺氧处理时,宫内缺氧子代大鼠血清AngⅡ睡眠显著低于宫内给予常氧的子代大鼠(2.07±0.17 pg/ml vs.2.50±0.34 pg/ml,P<0.05);而ADP水平显著高于后者(14.60±0.32 mg/L vs.11.51±1.98 mg/L,P<0.05)。结论孕期宫内缺氧能够改变子代大鼠的激素水平,导致AngⅡ水平明显升高,而ADP水平明显降低;缺氧能够改变机体的激素水平,在子代继续给予缺氧处理的大鼠其AngⅡ水平明显降低;而ADP却升高。
Objective To investigate the effects of chronic hypoxia on the serum levels of angiotensin Ⅱ (Ang Ⅱ) and adiponectin (ADP) in offspring in utero and postnatal rats. Methods Twenty SD female SD rats were randomly divided into two groups: chronic intermittent hypoxia group (I) and normoxia group (N). Male offspring were randomly divided into 3 groups (C) and chronic intermittent hypoxia group (H). Hypoxia group was given hypoxic treatment according to chronic intermittent hypoxia model. The hypoxia was induced for 8 hours daily, , Offspring rats were treated for 12 weeks after birth. Normoxia routine feeding. At the end of the experiment, sera of offspring rats were collected, serum Ang Ⅱ was detected by ELISA, and plasma adiponectin (ADP) was measured by sandwich enzyme-linked immunosorbent assay. Results When normoxia was given to offsprings, the level of serum AngⅡ in the rats with hypoxia in uterus was significantly higher than that in the neonates fed with normoxia (2.39 ± 0.45 pg / ml vs.2.06 ± 0.12 pg / ml, P <0.05), while the level of ADP was significantly lower than the latter (12.03 ± 1.14 mg / L vs 14.15 ± 1.72 mg / L, P <0.05). When the offspring rats were given hypoxia treatment, the serum Ang Ⅱ sleep in hypodermic rats was significantly lower than that in intrauterine administration of normal oxygen (2.07 ± 0.17 pg / ml vs.2.50 ± 0.34 pg / ml , P <0.05), while the level of ADP was significantly higher than the latter (14.60 ± 0.32 mg / L vs.11.51 ± 1.98 mg / L, P <0.05). Conclusion Intrauterine hypoxia can change hormone levels in offspring rats during pregnancy, resulting in a significant increase of AngⅡ level and a significant decrease of ADP level. Hypoxia can change hormone levels in the offspring and continue hypoxic treatment in offspring AngⅡ level decreased significantly; while ADP increased.