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目的制备粒径符合要求(200 nm以内)的反义寡核苷酸-聚丙交酯乙交酯纳米粒(ATM-ASODNs-PLGA-NP),并初步测定纳米粒的一些理化性质。方法以无毒的可生物降解的高分子材料聚丙交酯乙交酯作为载体材料,采用双乳化溶媒蒸发法制备载ATM-ASODNs-PLGA-NP,并评价其粒子形态、多分散性、包封率和进入人喉颈癌细胞(Hep-2)的能力等。结果制备的纳米粒形态圆整,大小均匀,平均粒径为87.9 nm,PDI为0.116,平均包封率为81.70%。经过PLGA纳米粒的包裹,对ATM-ASODN可起较好的保护作用,能载ATM-ASODN进入Hep-2细胞。结论载反义寡核苷酸纳米粒的制备工艺简便,粒子性状符合要求。
OBJECTIVE To prepare ATM-ASODNs-PLGA-NP (antisense oligodeoxynucleotide-PLGA-NP) with satisfactory particle size (within 200 nm) and to determine some physicochemical properties of nanoparticles. Methods The non-toxic biodegradable poly (lactide-co-glycolide) as carrier material was prepared by double-emulsion solvent evaporation method. The morphology, polydispersity and encapsulation of ATM-ASODNs-PLGA- Rate and access to human laryngeal carcinoma cells (Hep-2) and so on. Results The prepared nanoparticles were round and uniform in size with an average particle size of 87.9 nm and a PDI of 0.116 with an average entrapment efficiency of 81.70%. After the encapsulation of PLGA nanoparticles, ATM-ASODN can play a good protective effect, can carry ATM-ASODN into Hep-2 cells. Conclusion The preparation of antisense oligonucleotide nanoparticles is simple and the particle properties meet the requirements.