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目的了解TCRζ缺陷的急性髓细胞白血病(acute myeloid leukemia,AML)病人外周血T细胞中Elf-1、Zap-70和FcεR Iγ基因的表达特点。方法采用SYBR GreenⅠ荧光定量PCR和相对定量分析法检测10例TCRζ缺陷的AML病人外周血CD3+T细胞中Zap-70、Elf-1和FcεR Iγ基因的表达情况。以β2微球蛋白基因(β2M)作为内参,10例健康成人作为对照;采用相对定量公式:2-△Ct×100%,计算Zap-70、Elf-1和FcεR Iγ基因的表达水平。结果 TCRζ缺陷的AML病人外周血T细胞中Elf-1基因的表达水平(中位数8.526%)明显低于健康对照组(中位数33.237%)(P<0.001),而FcεR Iγ基因的表达水平(中位数2.571%)则高于健康对照组(中位数0.958%)(P=0.019),Zap-70基因在两组中的表达水平没有显著性差异,但均与TCRζ基因的表达水平呈正相关。结论 TCRζ缺陷的AML病人T细胞中Elf-1基因的低表达可能是导致AML病人TCRζ基因缺陷性表达的原因之一,而FcεR Iγ基因的高表达则可能是在一定程度上缓解了TCRζ基因缺陷性表达所带来的T细胞免疫异常。
Objective To investigate the expression of Elf-1, Zap-70 and FcεR Iγ in peripheral blood T cells of TCRζ-deficient acute myeloid leukemia (AML) patients. Methods The expression of Zap-70, Elf-1 and FcεR Iγ in peripheral blood CD3 + T cells of 10 TCRζ-deficient AML patients were detected by SYBR GreenⅠ fluorescence quantitative PCR and relative quantitative analysis. Β2 microglobulin gene (β2M) was used as an internal control, and 10 healthy adults were used as controls. The relative quantification formula was used to calculate the expression levels of Zap-70, Elf-1 and FcεR Iγ genes by 2- △ Ct × 100%. Results The expression of Elf-1 gene (median 8.526%) in TCRζ-deficient AML patients was significantly lower than that in healthy controls (median 33.237%) (P <0.001), while the expression of FcεR Iγ (Median 2.571%) was higher than that of healthy controls (median 0.958%) (P = 0.019). There was no significant difference in the expression level of Zap-70 between the two groups, but both were correlated with the expression of TCRζ The level was positively correlated. Conclusions The low expression of Elf-1 gene in T cells of AML patients with TCRζ deficiency may be responsible for the defective expression of TCRζ gene in AML patients. However, the overexpression of FcεR Iγ gene may partly alleviate the TCRζ gene defect T cell immune dysfunction caused by sexual expression.