D-myo-肌醇-1,4,5-三磷酸(IP_3)是许多动物细胞内重要的第二信使之一。分离和检测IP_3对研究多种递质或激素的作用机制和生理功能都有重要的意义。由于IP_3是一种环醇碳水化合物,没有生色团、荧光团或电活性基团,因而不能直接用高效液相色谱(HPLC)-紫外、荧光或电化学检测法来分析。然而在一定条件下,IP_3可被碱性磷酸酶水解产生无机磷酸、磷酸又迅速与酸性钼酸铵 D-myo-inositol-1,4,5-triphosphate (IP_3) is one of the important second messengers in many animal cells. Separation and detection of IP_3 have important implications for the study of the mechanisms and physiological functions of various neurotransmitters or hormones. Since IP_3 is a cycloaliphatic carbohydrate and has no chromophore, fluorophore or electroactive groups, it can not be analyzed directly by high performance liquid chromatography (HPLC) - UV, fluorescence or electrochemical detection. However, under certain conditions, IP_3 can be hydrolyzed by alkaline phosphatase to produce inorganic phosphoric acid which in turn rapidly interacts with acidic ammonium molybdate