论文部分内容阅读
目的:研究骨桥蛋白在人胸主动脉瘤(TAA)和腹主动脉瘤(AAA)中的表达及其意义。方法:收集TAA标本13例、AAA标本20例以及正常主动脉标本6例,用免疫组织化学法检测其中骨桥蛋白的表达;并对其中的TAA标本8例、AAA标本10例及正常主动脉标本6例用Western blot法检测其中骨桥蛋白的表达。结果:在免疫组化实验和Western blot实验中,骨桥蛋白在TAA组和AAA组中均呈现阳性表达,而在正常对照组中为阴性,TAA组和AAA组与正常对照组比较差异有统计学意义(P<0.05),TAA组与AAA组之间的表达差异无统计学意义(P>0.05)。结论:骨桥蛋白与人TAA及AAA的发病之间存在着相关性。
Objective: To study the expression and significance of osteopontin in human thoracic aortic aneurysm (TAA) and abdominal aortic aneurysm (AAA). Methods: 13 cases of TAA, 20 cases of AAA and 6 cases of normal aorta were collected. The expression of osteopontin was detected by immunohistochemical method. Among them, 8 cases of TAA, 10 cases of AAA and normal aorta Six specimens were detected by Western blot osteopontin expression. Results: In immunohistochemistry and Western blot, osteopontin showed positive expression in TAA group and AAA group, but negative in normal control group, there was statistical difference between TAA group and AAA group and normal control group (P <0.05). There was no significant difference between TAA group and AAA group (P> 0.05). CONCLUSIONS: There is a correlation between osteopontin and the incidence of human TAA and AAA.