目的　采用HPLC法测定新疆大花罗布麻叶中罗布麻甲素的含量。方法　SupelcosilTMLC18色谱柱 (4.6mm× 2 5 0mm ,5μm) ;流动相为甲醇 - 0 .1%磷酸水溶液 (40∶6 0 ) ;流速 1ml·min-1;柱温 35℃ ;检测波长 35 8nm。结果　罗布麻甲素和其他组分可达基线分离 ,线性范围 0 .0 2 1～ 0 .12 6 μg(r=0 .9995 ) ,平均回收率为 96 .4 % ,方法精密度的RSD =2 .8% (n =6 )。 结论　所用方法操作简便快速、准确灵敏 ,分离效果好。 Objective To determine the content of apocynin in Xinjiang Apocynum macrocephala leaves by HPLC. Methods SupelcosilTM LC18 column (4.6mm×250mm, 5μm) was used as the mobile phase. The mobile phase consisted of methanol-0.1% phosphoric acid aqueous solution (40∶60); flow rate was 1ml·min-1; column temperature was 35°C; detection wavelength was 35 8nm. Results The apocynin and other components were separated from the baseline with a linear range of 0.021 to 0.112 6 μg (r=0.9995) with an average recovery of 96.4%. RSD of method precision 2.8% (n = 6). Conclusion The method used is simple, rapid, accurate, and sensitive. The separation effect is good.