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对分离来自华南红树林的溶磷菌进行16SrDNA或ITS等基因水平上的初步鉴定,测定其溶解无机磷的能力,并对溶磷菌的生长培养基条件进行优化。结果表明,溶磷真菌的溶磷效果明显强于溶磷细菌,且溶磷真菌的溶解无机磷能力与培养液的pH呈极显著负相关,而溶磷细菌的溶磷能力与pH没显著相关关系。单因素实验表明,对供试菌株生长的合适碳源为麦芽糖,氮源为尿素。通过正交实验得到的优化培养基为麦芽糖5g/L、尿素0.05g/L、NaCl5g/L、pH5,在30°C下培养48h菌落总数可达6.06×109CFU/mL。
The isolation of phosphorus-solubilizing bacteria from mangrove forests in South China was carried out on 16S rDNA or ITS genes. The ability of dissolving inorganic phosphorus was determined and the conditions of growth medium for phosphate-solubilizing bacteria were optimized. The results showed that the phosphate solubilizing effect of the phosphate solubilizing fungi was significantly stronger than that of the phosphate solubilizing bacteria, and the dissolubility ability of the phosphate solubilizing fungi was significantly and negatively correlated with the pH of the solution. However, the ability of dissolving phosphorus of the phosphate solubilizing bacteria was not significantly correlated with the pH relationship. The single factor experiments showed that the suitable carbon source for the growth of the tested strains was maltose and the nitrogen source was urea. The optimal culture medium obtained by orthogonal experiment was 5g / L maltose, 0.05g / L urea, NaCl5g / L, pH5, and the total number of colonies cultured at 30 ° C for 48h reached 6.06 × 109CFU / mL.