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目的研究荔枝核总黄酮(TFL)对转化生长因子-β1(TGF-β1)诱导的人肝星状细胞(HSC-LX2)增殖与核因子-k B(NF-кB)、α-平滑肌肌动蛋白(α-SMA)表达的影响。方法体外培养HSC-LX2,TGF-β1刺激24 h后给予不同浓度的TFL(80、160、320、640、800μg/ml)干预;给药后24、48、72 h,用MTT法分别检测TFL对HSC-LX2增殖的影响;采用半定量PCR检测各组NF-кB、α-SMA mRNA的表达;Western blot检测NF-кB、α-SMA蛋白的表达情况。结果 MTT检测结果显示TFL呈时间依赖性抑制TGF-β1诱导HSC-LX2增殖,以作用48 h最为明显,其半数抑制浓度(IC50)为302μg/ml。TFL各剂量组作用48 h后,HSC-LX2细胞中NF-кB和α-SMA基因和蛋白的表达水平都降低,尤以300μg/ml和600μg/ml药物浓度组较明显,与对照组比较,有显著性差异(P<0.05)。结论 TFL在体外对TGF-β1诱导的HSC-LX2的增殖有抑制作用,其作用机制可能是通过抑制细胞中NF-кB的表达,从而起到抗肝纤维化的作用。
Objective To study the effects of total flavonoids of litchi core (TFL) on the proliferation and nuclear factor-kappa B (NF-κB), α-smooth muscle activity of human hepatic stellate cells (HSC-LX2) induced by transforming growth factor-β1 (TGF- Protein (α-SMA) expression. Methods HSC-LX2 and TGF-β1 were stimulated by TFL (80, 160, 320, 640 and 800μg / ml) in different concentrations for 24 h. Twenty-four, 48 and 72 h after the administration, TFL On the proliferation of HSC-LX2. The expressions of NF-κB and α-SMA mRNA in each group were detected by semi-quantitative PCR. The expressions of NF-κB and α-SMA protein were detected by Western blot. Results MTT assay showed that TFL inhibited the proliferation of HSC-LX2 induced by TGF-β1 in a time-dependent manner, with the most obvious effect at 48 h. The IC50 of TFL was 302 μg / ml. The expression of NF-кB and α-SMA gene and protein in HSC-LX2 cells decreased after each dose of TFL for 48 h, especially in the drug concentration of 300μg / ml and 600μg / ml. Compared with the control group, There was significant difference (P <0.05). Conclusion TFL can inhibit the proliferation of HSC-LX2 induced by TGF-β1 in vitro. The possible mechanism is that TFL can inhibit the expression of NF-κB in the cells and thus play an anti-hepatic fibrosis effect.