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本研究中,利用差异显示法,在碳酸盐逆境下,从水稻(OryzasativaL.)根的cDNA文库中克隆得到了水稻热激蛋白90基因(rHsp90,GenBankAccessionNo.AB037681)。Northern杂交结果表明,在水稻根中,rHsp90基因的转录水平在包括盐(NaCl,NaHCO3和Na2CO3),PEG,高pH(pH8.0和pH11.0)以及热激(50℃)逆境下,都受到了不同程度的诱导。同时,适量表达rHsp90基因的酵母(Saccharomycescerevisiae)在各种盐逆境以及热激逆境中,生长状态要好于对照。对转水稻rHsp90基因烟草的抗盐(NaCl,NaHCO3)分析表明,转基因烟草的生长势要好于野生型烟草。通过以上研究表明,水稻rHsp90基因与逆境之间具有一定的应答关系,并在植物适应环境逆境过程中起着重要的作用。
In this study, the rice heat shock protein 90 gene (rHsp90, GenBank Accession No. AB037681) was cloned from the cDNA library of rice (Oryzasativa L.) root under differential stress using the differential display method. Northern blot results showed that in the rice roots, the transcriptional level of rHsp90 gene was significantly higher in the stress environment including salt (NaCl, NaHCO3 and Na2CO3), PEG, high pH (pH8.0 and pH11.0) and heat shock (50 ℃) Has been induced to varying degrees. At the same time, yeast (Saccharomyces cerevisiae), which overexpress rHsp90 gene, grew better in all kinds of salt stress and heat shock stress than the control. Salt tolerance (NaCl, NaHCO3) analysis of rHsp90 transgenic rice showed that the growth potential of transgenic tobacco was better than that of wild type tobacco. The above results show that rHsp90 gene in rice has a certain response relationship with stress and plays an important role in plant adaptation to environmental stress.