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目的检测睡眠剥夺对荷瘤小鼠血液中miR-145的表达及肿瘤进展的影响。方法将无胸腺裸鼠随机分成正常-睡眠剥夺组、正常-非睡眠剥夺组、荷瘤-睡眠剥夺组、荷瘤-非睡眠剥夺组。通过皮下注射乳腺癌细胞建立荷瘤小鼠模型,利用多平台水环境法进行小鼠睡眠剥夺处理。利用卡尺测量肿瘤体积,免疫印迹法检测肿瘤组织血管内皮生长因子(VEGF)的表达,免疫组化检测新血管形成,并记录42 d内小鼠存活率。结果睡眠剥夺下调正常小鼠和荷瘤小鼠血液miR-145表达,与正常小鼠比较,荷瘤小鼠miR-145表达下调幅度更大。与荷瘤-非睡眠剥夺组相比,睡眠剥夺增加小鼠肿瘤体积和重量,促进肿瘤组织VEGF蛋白的表达和新生血管形成,并降低小鼠42 d内存活率。结论睡眠剥夺降低小鼠miR-145的表达并促进小鼠体内肿瘤进展。
Objective To detect the effect of sleep deprivation on the expression of miR-145 in blood and tumor progression in tumor-bearing mice. Methods Athymic nude mice were randomly divided into normal-sleep deprivation group, normal-non-sleep deprivation group, tumor-bearing sleep deprivation group and tumor-bearing non-sleep deprivation group. The tumor-bearing mouse model was established by injecting breast cancer cells subcutaneously, and the mouse sleep deprivation treatment was performed by the multi-platform water environment method. Tumor volume was measured by calipers. The expression of vascular endothelial growth factor (VEGF) in tumor tissue was detected by immunoblotting. The neovascularization was detected by immunohistochemistry and the survival rate of mice was recorded after 42 days. Results Sleep deprivation down-regulated the expression of miR-145 in normal mice and tumor-bearing mice. Compared with normal mice, miR-145 expression was down-regulated more in tumor-bearing mice. Compared with tumor-bearing-non-sleep deprivation group, sleep deprivation increased the tumor volume and weight of mice, promoted the expression of VEGF protein and neovascularization in tumor tissues and decreased the survival rate of mice in 42 d. Conclusion Sleep deprivation can reduce the expression of miR-145 in mice and promote tumor progression in mice.