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该研究是从露髓处收集牙髓血液标本,比较正常和炎症牙髓中免疫球蛋白(IgG,IgA和IgM),弹性蛋白酶,前列腺素E_2(PGE_2)、白细胞介素(IL-1α,IL-1β,IL-6)和肿瘤坏死因子-α(TNF-α)的水平。 材料和方法 选择9例正常牙髓,27例炎症牙髓,隔湿牙齿,暴露牙髓,将尼龙纤维棉球放置露髓处5s,吸收牙髓血液,用称重法算出血液标本量,然后用磷酸缓冲盐水(PBS,pH7.2)洗脱,稀释100倍,离心,取上清液备用。用酶联免疫吸收分析法(ELISA)的双抗体
In this study, we collected dental pulp blood samples from the demyelinated and compared the expression of immunoglobulin (IgG, IgA and IgM), elastase, prostaglandin E_2 (PGE_2), interleukin (IL-1α, IL) in normal and inflammatory pulp -1β, IL-6) and tumor necrosis factor-α (TNF-α). MATERIALS AND METHODS Nine cases of normal dental pulp, 27 cases of inflammatory dental pulp, wet teeth and dental pulp were exposed. The nylon fiber cotton balls were placed in the exposed pulp for 5 seconds to absorb the blood of the dental pulp. The blood samples were weighed and then Elute with phosphate buffered saline (PBS, pH 7.2), dilute 100 times, centrifuge, and take the supernatant for later use. Diabodies with enzyme-linked immunosorbent assay (ELISA)