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为了揭示温度这一重要环境因子与罗非鱼免疫力及对海豚链球菌的易感性之间的关系,分别进行了不同温度下罗非鱼感染海豚链球菌的死亡率试验和不同温度对罗非鱼非特异免疫相关酶(超氧化物歧化酶,SOD;溶菌酶;碱性磷酸酶,AKP以及补体C3)活性影响的试验。试验结果表明,罗非鱼感染海豚链球菌的死亡率与水温的变化呈现明显的正相关;特别是在高温条件下,罗非鱼免疫力受到了明显的抑制,具体表现为SOD活性呈现先诱导后抑制的趋势,AKP活性在12h较28℃时显著上升(P<0.05),血清溶菌酶活性则受到抑制作用,而补体C3活性在12h和24h与对照组相比显著升高了10.99%和13.40%(P<0.05)。试验结果显示高温能够引起罗非鱼免疫力低下,使鱼体对病原菌易感性增强,致使罗非鱼因感染海豚链球菌造成的死亡率显著升高,为研究罗非鱼海豚链球菌病爆发的环境机制提供了相关资料。
In order to reveal the relationship between the important environmental factor of temperature and tilapia immunity and the susceptibility to S. dolphin, the mortality of S. iniae infected with tilapia at different temperatures was tested, Test of the effect of non-specific immune-related enzymes (superoxide dismutase, SOD; lysozyme; alkaline phosphatase, AKP, and complement C3) on the activity of fish. The results showed that there was a significant positive correlation between mortality and water temperature of tilapia infected with Streptococcus eczema, especially in high temperature, the immunity of tilapia was significantly inhibited (P <0.05), while the activity of serum lysozyme was inhibited. However, the activity of complement C3 was significantly increased by 10.99% at 12h and 24h compared with that of the control group 13.40% (P <0.05). The results showed that high temperature can cause tilapia immunity is low, so that the fish susceptible to pathogens increased, resulting in tilapia infection caused by Streptococcus dolphin mortality was significantly increased, to study the occurrence of tilapia dolphin streptococcal disease The environmental mechanism provided relevant information.