[目的]探讨水稻水通道蛋白OsPIP2-6的亚细胞定位。[方法]克隆了水稻中的重要通道蛋白OsPIP2-6基因,构建了瞬时表达载体,通过基因枪法在洋葱表皮中进行瞬时表达,并通过激光共聚焦显微镜进行了观察。[结果]水稻水通道蛋白OsPIP2-6主要定位在细胞质膜上,证明了OsPIP2-6是个水通道蛋白。[结论]为植物水通道蛋白的深入研究提供了理论依据。 [Objective] The research aimed to investigate the subcellular location of rice aquaporin OsPIP2-6. [Method] OsPIP2-6 gene, an important channel protein in rice, was cloned. The transient expression vector was constructed and transiently expressed in onion epidermis by particle bombardment and observed by laser confocal microscopy. [Result] OsPIP2-6, a water channel protein of rice, was mainly localized on the plasma membrane and OsPIP2-6 was proved to be an aquaporin. [Conclusion] This provided a theoretical basis for the further study of aquaporin in plants.