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为了探讨血管紧张素Ⅱ的Ⅰ型受体(AT1R)拮抗剂氯沙坦(Losartan)的抗动脉粥样硬化(AS)作用及观察其是否能抑制单核细胞趋化蛋白-1(MCP-1)的蛋白及基因表达,我们通过高脂饮食及内皮损伤术建立兔AS模型,一组用Losartan(25mg/kg/d),另一组不用,喂养4个月,观察血管内膜的变化;同时做免疫组化及原位杂交,分别观察MCP-1蛋白及基因的表达。结果发现Losartan治疗组兔主动脉内膜面积及内、中膜面积之比均小于未治疗组,其MCP-1的蛋白及mRNA的表达亦明显减少,表明Losartan可通过抑制MCP-1的产生而具有抗AS作用。
To investigate the anti-atherosclerosis (AS) effect of losartan, an angiotensin II type 1 receptor (AT1R) antagonist, and to investigate whether it inhibits monocyte chemoattractant protein-1 ) Protein and gene expression, we established the rabbit AS model by high fat diet and endothelial injury. One group was treated with losartan (25mg / kg / d), the other group was fed for 4 months to observe the changes of intima; Immunohistochemistry and in situ hybridization were performed simultaneously to observe the expression of MCP-1 protein and gene. The results showed that the ratio of intimal area and intima-media area in the aorta of Losartan-treated rabbits was lower than that of the untreated group, and the protein and mRNA expression of MCP-1 in losartan group was also decreased significantly. It showed that Losartan could inhibit the production of MCP- With anti-AS effect.